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SDS-PAGE analyses of Endo H-treated immunoprecipitates derived from continuously labeled NTera2/D1 cells. Cells transiently transfected with pcDNA3, prohormones buy germany.1-WT, -Y21H, -P26L, or empty pcDNA3, prohormones buy germany.1 vector have been metabolically labeled for 18 h, prohormones buy germany. Asterisks indicate the migration of two unrelated proteins showing within the samples when pretreatment of the samples with Protein A Sepharose CL-4B and rabbit IgG was omitted, buy prohormones online uk.
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Cells transiently transfected with pcDNA3, buy prohormones canada0.1-WT , pcDNA3, buy prohormones canada0.1Y21H , or pcDNA3, buy prohormones canada0.1-P26L had been pulse labeled metabolically for 30 min and chased for the instances indicated above the lanes, buy prohormones canada0. Cell lysates and medium have been collected and immunoprecipitated with rabbit polyclonal anti-neurophysin II antiserum before separation on 15% Tris-HCl gels by SDS-PAGE and visualization using the PhosphorImager and ImageQuant software program. Cell lysates and medium from cells transfected with empty pcDNA3.1 vector have been analyzed in parallel as controls . The migration of molecular weight requirements is indicated on the left facet of the figures, buy prohormones canada1. The presumed identities of the precipitated products are indicated on the best facet of the figures.
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